Thursday, February 17, 2011
How Will Mechanomers Performing Desired Functions Be Detected in the Matrix?
Matricial analysis will of course use infrared spectroscopy and nuclear magnetic resonance imaging where appropriate. It will also use orthogonal analysis, by orthogonalization or third-dimensional separation of the matrix, for example by blotting the matrix into one end of and separating its components using a very wide chromatographic column (and orthogonal standards inoculated into the margin of the original square matrix marking in the orthogonal matrix or column planes or bands of interest). But matricial analysis will above all use mechanomeric indication, overlaying the matrix with a previously-mechanomerically-selected enzyme, an indicase, which under some condition resulting from the performance of the desired mechanomeric function catalyzes a reaction causing a color-change on the matrix. Such technique by its analysis at the molecular level, analysis by complexing, cumulative indication as colored indicator accumulates, and ability to use the product of one indicase to trigger another to amplify indication, will render most mechanomeric selection amenable to being performed as matricial mechanomeric selection. In such selection of any mechanomer the function of which is more complex than that of a simple enzyme catalyzing the indicating reaction, false indications will outnumber true, so multiple indicases should be developed and used to signal both true and false positives.